Impacts of household waste compost formed in public garbage dump on the organo-mineral status and productivity of a sandy soil

The inefficient use of mineral fertilizers on sandy soils in general has led users of this type of soil in Daloa (Cocircte d#39Ivoire), especially vegetable growers, to turn to other fertilizers more readily available such as household waste composted in open air. This study was conducted to evaluate the impact of the inputs of these residues on the fertility of the soils exploited by these producers. Thus, an experimental device was set up on lettuce according to farmers#39 conditions. This device consisted of four blocks each block was 21 m long. One block consisted of four elementary plots where different treatments were randomly distributed: the control did not receive compost and three additions of different compost doses (20, 40 and 60 t/ha). Some indicators of soil fertility, namely, soil organo-mineral status and the agricultural yield of the cultivation of lettuce carried out were evaluated. The results showed that the compost used had a significant positive effect on soil properties, and then, on the lettuce production when it was brought to soil at 40 and 60 t/ha. We concluded that solid waste compost can be an attractive alternative to chemical fertilizers on sandy soils

Modeling the contribution of ecological agriculture for climate change mitigation in cote d'Ivoire

The use of crop models is motivated by the prediction of crop production under climate change and for the evaluation of climate risk adaptation strategies. Therefore, in the present study the performance of DSSAT 4.6 was evaluated in a cropping system involving integrated soil fertility management options that are being promoted as ways of adapting agricultural systems to improve both crop yield and carbon sequestration on highly degraded soils encountered throughout middle Côte d’Ivoire. Experimental data encompassed two seasons in the Guinea savanna zone. Residues from the preceding vegetation were left to dry on plots like mulch on an experimental design that comprised the following treatments: (i) herbaceous savanna-maize, (ii)10 year-old of the shrub Chromolaena odorata fallow-maize (iii) 1 or 2 year-old Lalab pupureus stand-rotation, (iv) the legume L. pupureus -maize rotation; (v) continuous maize crop fertilized with urea; (vi) continuous maize crop fertilized with triple superphosphate; (vii) continuous maize crop, fertilized with both urea and triple superphosphate (TSP); (viii) continuous maize cultivation. The model’s sensitivity analysis was run to figure out how uncertainty of stable organic carbon (SOM3) can generate variation in the prediction of soil organic carbon (SOC) dynamics during the monitoring period of two years, within the first soil layer and to estimate the most suitable value. The observed variations were of 0.05 % in total SOC within the short-term and acceptable dynamics of changes were obtained for 0.80% of SOM3. The DSSAT model was calibrated using data from the 2007-2008 season and validated against independent data sets of yield of 2008-2009 to 2011-2012 cropping seasons. After the default values for SOM3 used in the model was substituted by the estimated one from sensitivity analysis, the model predicted average maize yields of 1 454 kg ha-1 across the sites versus an observed average value of 1 736 kg ha-1, R2 of 0.72 and RMSE of 597 kg ha-1. The impact of fallow residues and cropping sequence on maize yield was simulated and compared to conventional fertilizer and control data using historical climate scenarios over 12 years. Improving soil fertility through conservation agriculture cannot maintain grain yield in the same way as conventional urea inputs, although there is better yield stability against high climate variability according to our results

Seed systems smallholder farmers use

Seed can be an important entry point for promoting productivity, nutrition and resilience among smallholder farmers. While investments have primarily focused on strengthening the formal sector, this article documents the degree to which the informal sector remains the core for seed acquisition, especially in Africa. Conclusions drawn from a uniquely comprehensive data set, 9660 observations across six countries and covering 40 crops, show that farmers access 90.2 % of their seed from informal systems with 50.9 % of that deriving from local markets. Further, 55 % of seed is paid for by cash, indicating that smallholders are already making important investments in this arena. Targeted interventions are proposed for rendering formal and informal seed sector more smallholder-responsive and for scaling up positive impacts

Pronase E improves gene transduction of retinal ganglion cells

Poster Abstract #101Mark Hassall, Liu Guei-Sheung, Mammone Teresa, Wood John, Jack Ao, Alex Hewitt, Robert Casson, Jamie Craig, Glyn Chidlo

A novel small molecule inhibitor of human Drp1

Mitochondrial dynamin-related protein 1 (Drp1) is a large GTPase regulator of mitochondrial dynamics and is known to play an important role in numerous pathophysiological processes. Despite being the most widely used Drp1 inhibitor, the specificity of Mdivi-1 towards human Drp1 has not been definitively proven and there have been numerous issues reported with its use including off-target effects. In our hands Mdivi-1 showed varying binding affinities toward human Drp1, potentially impacted by compound aggregation. Herein, we sought to identify a novel small molecule inhibitor of Drp1. From an initial virtual screening, we identified DRP1i27 as a compound which directly bound to the human isoform 3 of Drp1 via surface plasmon resonance and microscale thermophoresis. Importantly, DRP1i27 was found to have a dose-dependent increase in the cellular networks of fused mitochondria but had no effect in Drp1 knock-out cells. Further analogues of this compound were identified and screened, though none displayed greater affinity to human Drp1 isoform 3 than DRP1i27. To date, this is the first small molecule inhibitor shown to directly bind to human Drp1

Preferentially Quantized Linker DNA Lengths in Saccharomyces cerevisiae

The exact lengths of linker DNAs connecting adjacent nucleosomes specify the intrinsic three-dimensional structures of eukaryotic chromatin fibers. Some studies suggest that linker DNA lengths preferentially occur at certain quantized values, differing one from another by integral multiples of the DNA helical repeat, ∼10 bp; however, studies in the literature are inconsistent. Here, we investigate linker DNA length distributions in the yeast Saccharomyces cerevisiae genome, using two novel methods: a Fourier analysis of genomic dinucleotide periodicities adjacent to experimentally mapped nucleosomes and a duration hidden Markov model applied to experimentally defined dinucleosomes. Both methods reveal that linker DNA lengths in yeast are preferentially periodic at the DNA helical repeat (∼10 bp), obeying the forms 10n+5 bp (integer n). This 10 bp periodicity implies an ordered superhelical intrinsic structure for the average chromatin fiber in yeast

Transcriptional Regulation of N-Acetylglutamate Synthase

The urea cycle converts toxic ammonia to urea within the liver of mammals. At least 6 enzymes are required for ureagenesis, which correlates with dietary protein intake. The transcription of urea cycle genes is, at least in part, regulated by glucocorticoid and glucagon hormone signaling pathways. N-acetylglutamate synthase (NAGS) produces a unique cofactor, N-acetylglutamate (NAG), that is essential for the catalytic function of the first and rate-limiting enzyme of ureagenesis, carbamyl phosphate synthetase 1 (CPS1). However, despite the important role of NAGS in ammonia removal, little is known about the mechanisms of its regulation. We identified two regions of high conservation upstream of the translation start of the NAGS gene. Reporter assays confirmed that these regions represent promoter and enhancer and that the enhancer is tissue specific. Within the promoter, we identified multiple transcription start sites that differed between liver and small intestine. Several transcription factor binding motifs were conserved within the promoter and enhancer regions while a TATA-box motif was absent. DNA-protein pull-down assays and chromatin immunoprecipitation confirmed binding of Sp1 and CREB, but not C/EBP in the promoter and HNF-1 and NF-Y, but not SMAD3 or AP-2 in the enhancer. The functional importance of these motifs was demonstrated by decreased transcription of reporter constructs following mutagenesis of each motif. The presented data strongly suggest that Sp1, CREB, HNF-1, and NF-Y, that are known to be responsive to hormones and diet, regulate NAGS transcription. This provides molecular mechanism of regulation of ureagenesis in response to hormonal and dietary changes

sFlt Multivalent Conjugates Inhibit Angiogenesis and Improve Half-Life In Vivo

We would like to thank Jonathan Winger and Xiao Zhu for guidance with the insect cell protein expression system and providing reagents. We would like to acknowledge Ann Fischer for help with expressing the sFlt protein in the Tissue Culture Facility at UC Berkeley and Dawn Spelke and Anusuya Ramasubramanian for help optimizing protein purification from insect cells. We are also grateful for the help from Leah Byrne and John Flannery at in the Helen Wills Neuroscience Institute at UC Berkeley for aiding us in the development of the rat intravitreal residence time model and for allowing us to use their facilities.Current anti-VEGF drugs for patients with diabetic retinopathy suffer from short residence time in the vitreous of the eye. In order to maintain biologically effective doses of drug for inhibiting retinal neovascularization, patients are required to receive regular monthly injections of drug, which often results in low patient compliance and progression of the disease. To improve the intravitreal residence time of anti-VEGF drugs, we have synthesized multivalent bioconjugates of an anti-VEGF protein, soluble fms-like tyrosine kinase-1 (sFlt) that is covalently grafted to chains of hyaluronic acid (HyA), conjugates that are termed mvsFlt. Using a mouse corneal angiogenesis assay, we demonstrate that covalent conjugation to HyA chains does not decrease the bioactivity of sFlt and that mvsFlt is equivalent to sFlt at inhibiting corneal angiogenesis. In a rat vitreous model, we observed that mvsFlt had significantly increased intravitreal residence time compared to the unconjugated sFlt after 2 days. The calculated intravitreal half-lives for sFlt and mvsFlt were 3.3 and 35 hours, respectively. Furthermore, we show that mvsFlt is more effective than the unconjugated form at inhibiting retinal neovascularization in an oxygen-induced retinopathy model, an effect that is most likely due to the longer half-life of mvsFlt in the vitreous. Taken together, our results indicate that conjugation of sFlt to HyA does not affect its affinity for VEGF and this conjugation significantly improves drug half-life. These in vivo results suggest that our strategy of multivalent conjugation could substantially improve upon drug half-life, and thus the efficacy of currently available drugs that are used in diseases such as diabetic retinopathy, thereby improving patient quality of life.Yeshttp://www.plosone.org/static/editorial#pee

Strategies and approaches to mangrove swamp rice varietal improvement in West Africa

(African Crop Science Journal, 1997 5(2): 209-217

Risk factors for chronic renal failure in Ivory coast: A prospective study of 280 patients

Chronic renal failure (CRF) represents the major cause of mortality in the nephro-logy unit in Ivory Coast because the means for appropriate management are lacking. The present study was performed to investigate the risk factors for CRF so that strategies for prevention could be elaborated. A case-control study was performed prospectively at the Yopougon Teaching Hos-pital in Abidjan from January 2006 to December 2006. Factors known to cause CRF were investigated in patients and controls. Their prevalence rates were compared with the general population. A total of 280 patients and 113 controls were recruited. The mean age of the patients was 37.88 ± 13.33 years and that of the controls was 41.5 ± 9.72 years. Both genders were equally represented. The main causes of CRF were chronic glomerulonephritis (47.48%), with HIV infec-tion accounting for 15% of them, and essential hypertension (HTA) (25%). Essential HTA repre-sented the only factor which, if untreated, inevitably leads to CRF. Thus, our study indicates that HTA is a major public health concern. All efforts should be implemented to reduce the high prevalence of HTA and the deleterious effect of this disorder in Ivory Coast